The advent of modern high throughput flow cytometry and high-powered microscopy methods now enable scientists and physicians to observe and depict cellular activities with unprecedented precision.
Such revolution in biotechnology demands the development of highly sensitive and robust cellular probes that faithfully report the location and turn-over of the enzymatic activities in a living cell with precise and long signal retention.
The bright fluorescence emitted by the SmartID probes when activated by their corresponding enzymes can be visualized or measured through various instruments such as epifluorescence or confocal microscopes, microplate readers and flow-cytometers. The simple utilization of SmartID probes allows for high-throughput analysis using classic laboratory instruments such as flow cytometers and microscopes.
SmartID technology can thus be used on most modern lab equipment. Heretofore unknown results can be obtained by fusing high-end, single-cell hardware (flow and scanning FACS machines) with the unique performance of SmartID probes.
Microplate reader (EnSpire 3500)
Microplate readers are a laboratory instrument used to measure chemical, biological or physical reactions, properties, and analytes within the well of a microplate. These reactions convert the presence of an analyte or the progression of biochemical processes into optical signals such as fluorescence or luminescence. These signals can then be detected by the microplate reader to quantifies the parameter of interest. The EnSpire 3500 is a configurable plate reader including the latest technological innovations, for detection by light absorption or emission (fluorescence intensity, luminescence, …).
LSRII flow cytometer
Flow cytometry is a technology that is used to analyze the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. Cell components are fluorescently labeled and then excited by the laser to emit light at varying wavelengths. The BD LSR II is the first and only air-cooled, four-laser benchtop flow cytometer with the ability to acquire up to 18 colors.
Analysis of a mixed populations using the LSRII flow cytometer with a sub-population of fluorescent human cells vizualized in green, and a sub-population of non-fluorescent human cells vizualized in red in the DIVA software.